SpectraMax i3x Multi-Mode Microplate Reader

SpectraMax i3x Multi-Mode Microplate Reader

Western blot, imaging, and injectors on one configurable microplate reader

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Unlimited breadth of user-upgradeable application modules expands research capabilities

The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user-configurable detection modules, the reader provides you with the ability to explore cellular pathways and protein expression on one microplate reader.

Capture flash assays with ease

Capture flash assays with ease

The SpectraMax i3x Injector Cartridge with SmartInject® Technology expands your research capabilities to include flash-based applications including dual luciferase and ATP assays.

Reduce background noise

Reduce background noise

The cooled PMT reduces background noise allowing for a more sensitive, wide dynamic range in extremely low light.

Increase fluorescence performance

Increase fluorescence performance

Powerful combination of Xenon flash lamp and LEDs provides unmatched signal strength and superior sensitivity with Spectral Fusion™ Illumination.

SpectraMax i3x Multi-Mode Detection Platform


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User-configurable applications

User-configurable detection modules expand the reader’s detection capabilities to include AlphaScreen, Time-Resolved Fluorescence, HTRF, fast kinetics with injectors, western blot detection, and more.

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No-stain cell analysis

The StainFree™ Cell Detection Algorithm for brightfield cell segmentation enables cell counting and confluency measurement without destructive stains, simplifying the cell counting workflow.

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Multi-channel functionality

Two fluorescence detection channels on the MiniMax cytometer enable the analysis of cell viability or cell toxicity assays, including ratiometric assays like live-dead and transfection efficiency.

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Cellular pathway analysis

Cell confluence and cell viability imaging, western blot analysis, and quantitation of nucleic acids and protein, can be captured on one reader.

Add Cellular Imaging to your Plate Reader Workflow

Observe the viability of your cells with the field-upgradeable cellular imaging option for the SpectraMax i3x Multi-mode Detection Platform.

The field-upgradeable SpectraMax® MiniMax™ 300 Imaging Cytometer enables quick imaging and analysis of cells and gives you a front-row view of phenotypic changes that accompany cytotoxicity, cell proliferation, and protein expression. Now with StainFree™ Cell counting Technology, additional brightfield analysis features, and fluorescent green and red detection channels.

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ETAP Lab use the SpectraMax i3x


ETAP Lab use the SpectraMax i3x to advance neurodegenerative disease research

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Applications of SpectraMax i3x Multi-Mode Microplate Reader

Specifications & Options of SpectraMax i3x Multi-Mode Microplate Reader

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SpectraMax® i3x Multi-Mode Microplate Reader

SpectraMax® i3x Multi-Mode Microplate Reader System

System Options
Part Number
SpectraMax i3x Multi-Mode Microplate Reader with SoftMax Pro Software
SpectraMax MiniMax 300 Imaging Cytometer
SoftMax Pro 7.x GxP Software
by license bundle
SpectraTest (ABS2) Absorbance Validation Plate
SpectraTest (LM1) Luminescence Validation Plate
SpectraTest (FL1) Fluorescence Validation Plate
SpectraDrop Micro-Volume Starter Kit
SpectraDrop Micro-Volume HTS Kit
SpectraMax Detection Cartridge Options
Product Name
Part Number
AlphaScreen Detection Cartridge (384 STD)
AlphaScreen Detection Cartridge (384 HTS)
AlphaScreen Detection Cartridge (1536 HTS)
HTRF Detection Cartridge
TRF Detection Cartridges

Fluorescence Polarization Detection Cartridge


Fluorescence Polarization Detection Cartridge (Rhodamine)

Luminescence Detection Cartridge

(Glow for 96-well plates)


Luminescence Detection Cartridge

(Glow 96- and 384-well plates)


Luminescence Detection Cartridge

(Glow 96-, 384-, and 1536-well plates)


Dual Glow Luminescence Detection Cartridge



Fluorescence Intensity Detection Cartridge



Fluorescence Intensity Detection Cartridge



Fluorescence Intensity Detection Cartridge


ScanLater Western Blot Detection Cartridge
SpectraMax Injector Cartridge with SmartInject™ Technology