The DispenCell Single-Cell Dispenser revolutionizes single-cell isolation by utilizing advanced microfluidics technology to accurately control the dispensing of individual cells. The tool can effectively detect, size, and dispense particles down to the single-particle resolution based on impedance change that depends on particle volume. It can accommodate a wide range of cell sizes and types, allowing researchers to adjust parameters such as cell concentration and dispensing volume at a gentle pressure of less than 0.1psi to achieve the desired cell density and distribution. This automation eliminates tedious manual labor, allowing researchers to focus on more complex tasks while significantly increasing throughput.