IC 50 Curves for Cytotoxic Compounds

HeLa cells were treated with anisomycin (red circles) or staurosporine (blue squares). Results were plotted using the 4-parameter curve fit in SoftMax® Pro software. IC50 for anisomycin was 3.3 μM, and IC50 for staurosporine was 0.53 μM; both values were consistent with published values.