HTRF cAMP assays, Gαi/o-coupled receptors

Cyclic AMP (cAMP, cyclic adenosine 3’, 5’–monophosphate) is a key second messenger in GPCR signaling. The generation of cAMP is probably the most studied of signal transduction pathways and is involved in responses to sensory input, hormones, nerve transmission, and much more. Upon ligand binding to a GPCR, a conformational change occurs, activating the receptor and in turn activating a G protein. Activation of Gαi/o–coupled receptors inhibits adenyl cyclase from generating cAMP.

Explore our resources for Gαi/o

• Application Note: HTRF cAMP dynamic 2 and IP-One assays on the SpectraMax M5e reader
• Poster: Homogeneous High Throughput Live Cell GPCR Functional and Surface Binding Assays