Fluorescence intensity changes with increase or decrease in cellular membrane potential

FLIPR Membrane Potential Assay Kits detect ion channel modulation by increasing or decreasing the fluorescent signal as cellular membrane potential changes. The fluorescent signal increases in intensity during membrane depolarization as dye follows the positively charged ions inside the cell. During membrane hyperpolarization, fluorescent signal decreases in intensity as dye follows the positively charged ions out of the cell. The kits are uniquely suited for use with the simultaneous pipet and read capability of the FLIPR® Tetra System and FlexStation® 3 Microplate Readers to capture fast kinetics associated with ion channel activation.

The kits employ a quenching dye to reduce background fluorescence and improve the signal-to-noise ratio. The patented quench technology (U.S. patent number 6,420,183, EPO patent number 0 906 572) is offered to drug discovery and life science researchers exclusively by Molecular Devices through the purchase of FLIPR Assay Kits.