Dated: Sep 01, 2022
Publication Name: Synthetic and Systems Biotechnology
Citations : Enhancement and mapping of tolerance to salt stress and 5-fluorocytosine in synthetic yeast strains via SCRaMbLE
Varied environmental stress can affect cell growth and activity of the cellular catalyst. Traditional path of adaptive evolution generally takes a long time to achieve a tolerance phenotype, meanwhile, it is a challenge to dissect the underlying genetic mechanism. Here, using SCRaMbLE, a genome scale tool to generate random structural variations, a total of 222 evolved yeast strains with enhanced environmental tolerances were obtained in haploid or diploid yeasts containing six synthetic chromosomes.
Contributors: Jianping Kang a b, Jieyi Li a b, Zhou Guo a b, Sijie Zhou a b, Shuxin Su a b, Wenhai Xiao a b, Yi Wu a b, and Yingjin Yuan