Colony Forming Assay

The soft agar colony formation assay is a classical and renowned technique for characterizing the ability of cells to undergo anchorage-independent growth, a hallmark of carcinogenesis. After seeding in a matrix that enhances colony formation, such as a semi-solid media, cells are typically incubated with compounds that may affect colony growth. The CloneSelect Imager can image every well and automatically calculate confluence, estimate colony number and area, and track colony growth.