Cell line development

Cell line development requires the discovery of single cell-derived clones that produce high and consistent levels of the target therapeutic protein with desired quality attributes, possible to be scaled up for commercial GMP production.

Here’s a few application notes on cell line development to aid in your COVID-19 vaccine research.

• Colony picking - Enhanced development of virus-specific hybridomas using ClonePix and CloneSelect Imager Technologies
• Monoclonality verification - Confident assurance of clonality using calcein AM with minimal effect on viability
• Cell sorting - A workflow combining single-cell isolation and microplate imaging improves cloning efficiency with higher assurance of clonality
• Cell line knock-ins/outs - Validate CRISPR-Edited Cells using Imaging and Western Blot Detection on a Microplate Reader