Calcium and IP-One assays (GPCR, Gq coupled)

Cell signaling via G protein-coupled receptors (GPCRs) can be assessed by monitoring the downstream effectors calcium or cyclic AMP (cAMP). Calcium flux in response to Gq protein-coupled receptor activation is commonly monitored in live cells in real time using calcium-sensitive dyes on a fluorescence plate reader. Another arm of this pathway is monitored using a TR-FRET assay for IP1.

Here are a few application notes you may find of interest:

• Monitoring Gq protein-coupled receptor activation on the SpectraMax i3x reader with injector module
• Optimization of a muscarinic M3-receptor assay using frozen CHO cells on the FlexStation 3 reader
• Calcium signaling with FLIPR Calcium 6 and 6-QF Assay Kits on the FlexStation 3 reader
• HTRF IP-One Gq assay on SpectraMax readers