Fluorometric quantitation of nucleic acids

Quantitation of DNA is a critical step in molecular biology requiring accuracy, reliability, and the use of increasingly smaller sample volumes for applications such as next-generation sequencing. Compared to spectrophotometric DNA quantitation, the fluorometric method provides key advantages such as significantly increased sensitivity, high selectivity for double-stranded DNA (dsDNA) over single-stranded DNA (ssDNA) or RNA, and improved contaminant tolerance (protein and carbohydrate molecules).

Here are a few application notes on fluorometric quantitation of nucleic acids that you may find of interest:

• Quantitation of dsDNA samples using the SpectraMax Quant dsDNA Assay Kits
• Quantitating Double-Stranded DNA with Quant-iT PicoGreen dsDNA Reagent and SpectraMax Fluorescence Microplate Readers